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Trans-Spliced Exon Coupled RNA End Determination : ウィキペディア英語版 | Trans-Spliced Exon Coupled RNA End Determination Trans-Spliced Exon Coupled RNA End Determination (TEC-RED) is a technique designed by Muller et al. that, like SAGE, allows for the digital detection of messenger RNA sequences. Unlike SAGE, detection and purification of transcripts from the 5’ end of the messenger RNA require the presence of a trans-spliced leader sequence. == Trans-splicing Background==
Spliced leader sequences are short sequences of non coding RNA, not found within a gene itself, that are attached to the 5’ end of all, or a portion of, mRNAs transcribed in an organism. They have been found in several species to be responsible for separating polycistronic transcripts into single gene mRNAs, and in others to splice onto monocistronic transcripts. The major role of trans-splicing on monocistronic transcripts is largely unknown. It has been proposed that they may act as an independent promoter that aids in tissue specific expression of independent protein isoforms. Spliced leaders have been seen in trypanosomatids, Euglena, flatworms, Caenorhabditis.〔 Some species contain only one spliced leader sequence found on all mRNAs. In C. elegans two are seen and are labeled SL1 and SL2.
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